abstract：:Ergosterol (a fungal membrane component) was shown to induce transient influx of protons and membrane hyperpolarization in cotyledonary cells of Mimosa pudica L. By contrast, chitosan (a fungal wall component with known elicitor properties) triggered membrane depolarization. In the processes induced by ergosterol, a s...

journal_title：Journal of experimental botany

authors： Rossard S,Luini E,Pérault JM,Bonmort J,Roblin G

更新日期：2006-01-01 00:00:00

abstract：:Most plant oxylipins, a large class of diverse oxygenated polyunsaturated fatty acids and their derivatives, are produced through the lipoxygenase (LOX) pathway. Recent progress in dicots has highlighted the biological roles of oxylipins in plant defence responses to pathogens and pests. By contrast, the physiological...

journal_title：Journal of experimental botany

authors： Nemchenko A,Kunze S,Feussner I,Kolomiets M

更新日期：2006-01-01 00:00:00

abstract：:The phloem is a well-known target of sucking and piercing insects that utilize the transported fluid as their major nutrient source. In addition to small molecules like sugars and amino acids, phloem sap of higher land plants contains proteins that can accumulate up to high concentrations. Although the knowledge about...

journal_title：Journal of experimental botany

authors： Kehr J

更新日期：2006-01-01 00:00:00

abstract：:Photo-oxidative stress was imposed under natural solar radiation on exposed and shaded sections of detached fruit of immature green tomato (Lycopersicon esculentum Miller = Solanum lycopersicum L.) mutants (anthocyanin absent, beta-carotene, Delta, and high pigment-1) and their nearly isogenic parents ('Ailsa Craig' a...

journal_title：Journal of experimental botany

authors： Torres CA,Andrews PK,Davies NM

更新日期：2006-01-01 00:00:00

abstract：:SPICK2, a homologue of the weakly-inward-rectifying Shaker-like Arabidopsis K channel, AKT2, is a candidate K+-influx channel participating in light- and clock-regulated leaf movements of the legume, Samanea saman. Light and the biological clock regulate the in situ K+-influx channel activity differentially in extenso...

journal_title：Journal of experimental botany

authors： Yu L,Becker D,Levi H,Moshelion M,Hedrich R,Lotan I,Moran A,Pick U,Naveh L,Libal Y,Moran N

更新日期：2006-01-01 00:00:00

abstract：:Plasma membrane vesicles isolated by two-phase partitioning from the storage root of Beta vulgaris show atypically high water permeability that is equivalent only to those reported for active aquaporins in tonoplast or animal red cells (Pf=542 microm s(-1)). The values were determined from the shrinking kinetics measu...

journal_title：Journal of experimental botany

authors： Alleva K,Niemietz CM,Sutka M,Maurel C,Parisi M,Tyerman SD,Amodeo G

更新日期：2006-01-01 00:00:00

abstract：:The Arabidopsis thaliana ascorbate-deficient vtc-1 mutant has only 30% ascorbate contents of the wild type (WT). This ascorbate-deficient mutant was used here to study the physiological roles of ascorbate under salt stress in vivo. Salt stress resulted in a more significant decrease in CO2 assimilatory capacity in the...

journal_title：Journal of experimental botany

authors： Huang C,He W,Guo J,Chang X,Su P,Zhang L

更新日期：2005-12-01 00:00:00

abstract：:In this study, the uptake of Na+ into the cytosol of rice (Oryza sativa L. cvs Pokkali and BRRI Dhan29) protoplasts was measured using the acetoxy methyl ester of the fluorescent sodium-binding benzofuran isopthalate, SBFI-AM, and fluorescence microscopy. By means of inhibitor analyses the mechanisms for uptake and se...

journal_title：Journal of experimental botany

authors： Kader MA,Lindberg S

更新日期：2005-12-01 00:00:00

abstract：:Plant stress studies are more and more based on gene expression. The analysis of gene expression requires sensitive, precise, and reproducible measurements for specific mRNA sequences. Real-time RT-PCR is at present the most sensitive method for the detection of low abundance mRNA. To avoid bias, real-time RT-PCR is r...

journal_title：Journal of experimental botany

authors： Nicot N,Hausman JF,Hoffmann L,Evers D

更新日期：2005-11-01 00:00:00

abstract：:The functions of two genes whose expression provides tolerance to aluminium (Al) stress were investigated using plants and Saccharomyces cerevisiae (yeast): the Arabidopsis thaliana blue copper binding gene (AtBCB) and Nicotiana tabacum guanosine diphosphate (GDP) dissociation inhibitor gene (NtGDI1). To determine the...

journal_title：Journal of experimental botany

authors： Ezaki B,Sasaki K,Matsumoto H,Nakashima S

更新日期：2005-10-01 00:00:00

abstract：:Flower and inflorescence reversion involve a switch from floral development back to vegetative development, thus rendering flowering a phase in an ongoing growth pattern rather than a terminal act of the meristem. Although it can be considered an unusual event, reversion raises questions about the nature and function ...

journal_title：Journal of experimental botany

authors： Tooke F,Ordidge M,Chiurugwi T,Battey N

更新日期：2005-10-01 00:00:00

abstract：:With the general aim of elevating the content of the essential amino acid methionine in vegetative tissues of plants, alfalfa (Medicago sativa L.) and tobacco plants, as well as BY2 tobacco suspension cells, were transformed with a beta-zein::3HA gene under the 35S promoter of cauliflower mosaic virus encoding a rumen...

journal_title：Journal of experimental botany

authors： Amira G,Ifat M,Tal A,Hana B,Shmuel G,Rachel A

更新日期：2005-09-01 00:00:00

abstract：:Dihydroflavonol 4-reductase (DFR) is the first committed enzyme of the anthocyanin and condensed tannin pathways. Several DFR cDNAs have been cloned, and different specificities of DFR isozymes in the substrate hydroxylation patterns have been reported, but only fragmentary knowledge of DFR gene organization is availa...

journal_title：Journal of experimental botany

authors： Shimada N,Sasaki R,Sato S,Kaneko T,Tabata S,Aoki T,Ayabe S

更新日期：2005-09-01 00:00:00

abstract：:Growing potato tubers or freshly harvested mature tubers have a dormant apical bud. Normally, this dormancy is spontaneously broken after a period of maturation of the tuber, resulting in the growth of a new sprout. Here it is shown that in in vitro-cultured growing and maturing tubers, ethanol can rapidly break this ...

journal_title：Journal of experimental botany

authors： Claassens MM,Verhees J,van der Plas LH,van der Krol AR,Vreugdenhil D

更新日期：2005-09-01 00:00:00

abstract：:Populus euphratica is a poplar species growing in arid regions of Central Asia, where its distribution remains nevertheless restricted to river-banks or to areas with an access to deep water tables. To test whether the hydraulic architecture of this species differs from that of other poplars with respect to this ecolo...

journal_title：Journal of experimental botany

authors： Hukin D,Cochard H,Dreyer E,Le Thiec D,Bogeat-Triboulot MB

更新日期：2005-08-01 00:00:00

abstract：:Ozone induces rapid activation of SIPK, a mitogen-activated protein kinase (MAPK) in tobacco. Through transgenic manipulation it has previously been shown that overexpression of SIPK leads to enhanced ozone-induced lesion formation with concomitant accumulation of ROS. In spite of this hypersensitive phenotype, the ef...

journal_title：Journal of experimental botany

authors： Samuel MA,Walia A,Mansfield SD,Ellis BE

更新日期：2005-08-01 00:00:00

abstract：:Notwithstanding the economic importance of non-climacteric fruits like grape and strawberry, little is known about the mechanisms that regulate their ripening. Up to now no growth regulator has emerged with a primary role similar to that played by ethylene in the ripening of the climacteric fruits. Strawberries can pr...

journal_title：Journal of experimental botany

authors： Trainotti L,Pavanello A,Casadoro G

更新日期：2005-08-01 00:00:00

abstract：:A highly reproducible regeneration system through somatic embryogenesis from the excised mature embryos (MEs) of dry seeds of a range of European barley cultivars was developed. By minimizing the germination of plated MEs, primary callus could be obtained with high frequency which permitted efficient embryogenesis and...

journal_title：Journal of experimental botany

authors： Sharma VK,Hänsch R,Mendel RR,Schulze J

更新日期：2005-07-01 00:00:00

abstract：:The Arabidopsis thaliana THI1 protein is involved in thiamine biosynthesis and is targeted to both chloroplasts and mitochondria by N-terminal control regions. To investigate thi1 expression, a series of thi1 promoter deletions were fused to the beta-glucuronidase (GUS) reporter gene. Transgenic plants were generated ...

journal_title：Journal of experimental botany

authors： Ribeiro DT,Farias LP,de Almeida JD,Kashiwabara PM,Ribeiro AF,Silva-Filho MC,Menck CF,Van Sluys MA

更新日期：2005-07-01 00:00:00

abstract：:Four glutathione S-transferase (GST) genes, NbGSTU1, NbGSTU2, NbGSTU3, and NbGSTF1, were amplified from cDNA of Nicotiana benthamiana leaves infected with Colletotrichum destructivum using primers based on conserved regions of N. tabacum GST sequences. Expression of NbGSTU1 and NbGSTU3 increased progressively during i...

journal_title：Journal of experimental botany

authors： Dean JD,Goodwin PH,Hsiang T

更新日期：2005-06-01 00:00:00

abstract：:Although leaves and other vegetative tissues are generally considered as non-climacteric, citrus leaves show a climacteric system II behaviour after detachment. Upon harvest, young, fully expanded 'Valencia' orange (Citrus sinensis) leaves ( approximately 60-d-old) exhibited two phases of ethylene production. The firs...

journal_title：Journal of experimental botany

authors： Katz E,Riov J,Weiss D,Goldschmidt EE

更新日期：2005-05-01 00:00:00

abstract：:Apoplastic transport barriers in the roots of rice (Oryza sativa L. cv. IR64) and corn (Zea mays L. cv. Helix) were isolated enzymatically. Following chemical degradation (monomerization, derivatization), the amounts of aliphatic and aromatic suberin monomers were analysed quantitatively by gas chromatography and mass...

journal_title：Journal of experimental botany

authors： Schreiber L,Franke R,Hartmann KD,Ranathunge K,Steudle E

更新日期：2005-05-01 00:00:00

abstract：:A SAG12:ipt gene construct, which increases cytokinin biosynthesis in response to senescence, was introduced into Arabidopsis plants to delay senescence induced by flooding stress. Two forms of flooding stress, including total submergence and root waterlogging, were applied to SAG12:ipt (IPT) and wild-type (WT) plants...

journal_title：Journal of experimental botany

authors： Huynh le N,Vantoai T,Streeter J,Banowetz G

更新日期：2005-05-01 00:00:00

abstract：:Plant species capable of hyper-accumulating heavy metals are of considerable interest for phytoremediation, and differ in their ability to accumulate metals from the environment. This work aims to examine (i) arsenic accumulation in three fern species [Chinese brake fern (Pteris vittata L.), slender brake fern (Pteris...

journal_title：Journal of experimental botany

authors： Srivastava M,Ma LQ,Singh N,Singh S

更新日期：2005-05-01 00:00:00

abstract：:The effects of increasing light and of a heterologous bHLH transcription factor on the accumulation of condensed tannins (CT) were investigated in leaves of Lotus corniculatus, a model legume species which accumulates these secondary metabolites in leaves as well as reproductive tissues. Light and expression of the tr...

journal_title：Journal of experimental botany

authors： Paolocci F,Bovone T,Tosti N,Arcioni S,Damiani F

更新日期：2005-04-01 00:00:00

abstract：:Alloplasmic lines of Brassica napus with rearranged Arabidopsis thaliana mitochondrial DNA are male sterile and vegetatively altered compared with B. napus cv. Hanna. The CMS lines contain pure nuclear and plastid genomes from B. napus. Cross-sections of leaves revealed elevated starch accumulation and a higher number...

journal_title：Journal of experimental botany

authors： Teixeira RT,Knorpp C,Glimelius K

更新日期：2005-04-01 00:00:00

abstract：:An Arabidopsis thaliana cDNA (At-74) has been isolated that encoded an uncharacterized protein showing homology with members of the d-PGMase superfamily: cofactor-dependent phosphoglycerate mutases (d-PGM-ases) and the phosphatase domain of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatases (6PF2Kase/F2, 6Pase). Pr...

journal_title：Journal of experimental botany

authors： Bourgis F,Botha FC,Mani S,Hiten FN,Rigden DJ,Verbruggen N

更新日期：2005-04-01 00:00:00

abstract：:A gene MtPPRD1, encoding a protein of 132 amino acids containing a proline-rich domain (PRD), has been revealed by suppressive subtractive hybridization (SSH) with two mRNA populations of embryo axes harvested immediately before and after radicle emergence. Although at the protein level MtPPRD1 showed low homology wit...

journal_title：Journal of experimental botany

authors： Bouton S,Viau L,Lelièvre E,Limami AM

更新日期：2005-03-01 00:00:00

abstract：:The medicinally applied tropane alkaloids hyoscyamine and scopolamine are produced in Atropa belladonna L. and in a small number of other Solanaceae. Calystegines are nortropane alkaloids that derive from a branching point in the tropane alkaloid biosynthetic pathway. In A. belladonna root cultures, calystegine molar ...

journal_title：Journal of experimental botany

authors： Richter U,Rothe G,Fabian AK,Rahfeld B,Dräger B

更新日期：2005-02-01 00:00:00

abstract：:Dormancy of hazel (Corylus avellana L.) seeds is alleviated by a chilling treatment during which cytological, hormonal, and biochemical changes occur. Phytic acid and phosphate mobilization have been examined during this treatment. Phytic acid accounted for 0.7% and up to 3.2% of dry weight in axiferous and cotyledona...

journal_title：Journal of experimental botany

authors： Andriotis VM,Smith SB,Ross JD

更新日期：2005-02-01 00:00:00

abstract：:The spatial and temporal distribution of expression of two cytosolic members of the AtHsp90 gene family was assessed during early development. In stressed transgenic plants bearing the AtHsp90-3 promoter, beta-glucuronidase (GUS) activity was strong in meristematic tissues. Expression was also detected in vascular tis...

journal_title：Journal of experimental botany

authors： Prasinos C,Krampis K,Samakovli D,Hatzopoulos P

更新日期：2005-02-01 00:00:00

abstract：:Alpha-dioxygenase (alpha-DOX) enzymes catalyse the oxygenation of fatty acids to yield a newly identified group of oxylipins that play a role in protecting tissues from oxidative damage and cell death. In tomato (Lycopersicon esculentum Mill.) alpha-DOX was identified as salt-regulated by differential display of mRNA,...

journal_title：Journal of experimental botany

authors： Tirajoh A,Aung TS,McKay AB,Plant AL

更新日期：2005-02-01 00:00:00

abstract：:Water deficit during male meiosis in wheat (Triticum aestivum L.) causes pollen sterility. With a view to identifying the internal trigger for this failure, it was found that water stress specifically impairs the activities of vacuolar and cell-wall invertases in anthers prior to the arrest of pollen development. The ...

journal_title：Journal of experimental botany

authors： Koonjul PK,Minhas JS,Nunes C,Sheoran IS,Saini HS

更新日期：2005-01-01 00:00:00

abstract：:A cDNA clone (LeCRK1), encoding a novel isoform of calcium-dependent protein kinase (CDPK), was isolated by screening a tomato (Lycopersicon esculentum) cDNA library. The protein derived from the full-length sequence indicated that it belongs to the family of CDPK-related kinases (CRKs) and the predicted amino acid se...

journal_title：Journal of experimental botany

authors： Leclercq J,Ranty B,Sanchez-Ballesta MT,Li Z,Jones B,Jauneau A,Pech JC,Latché A,Ranjeva R,Bouzayen M

更新日期：2005-01-01 00:00:00

abstract：:One should perhaps start off by asking the question, 'But what wood is it we want to see?' There are so many trees that make up the wood; within a post-genomics context, genes, transcripts, proteins, and metabolites are the more tangible ones. Rather than studying these components in isolation, a more holistic approac...

journal_title：Journal of experimental botany

authors： Goodacre R

更新日期：2005-01-01 00:00:00

abstract：:Medicago truncatula is a model legume plant that interacts symbiotically with Sinorhizobium meliloti, the alfalfa symbiont. This process involves a molecular dialogue between the bacterium and the plant. Legume roots exude flavonoids that induce the expression of a set of rhizobial genes, the nod genes, which are esse...

journal_title：Journal of experimental botany

authors： Macchiavelli RE,Brelles-Mariño G

更新日期：2004-12-01 00:00:00

abstract：:The role of nitrate reduction to produce nitric oxide (NO) and its subsequent oxidation by oxyhaemoglobin as a mechanism to maintain plant cell energetics during hypoxia is examined. Nitrate reduction in hypoxic conditions can be considered as an alternative respiratory pathway, with nitrate as an intermediate electro...

journal_title：Journal of experimental botany

authors： Igamberdiev AU,Hill RD

更新日期：2004-12-01 00:00:00

abstract：:Quantitative genetics of adaptive traits is made difficult by the genotypexenvironment interaction. A classical assumption is that QTLs identified in both stressed and control conditions correspond to constitutive traits whereas those identified only in stressed treatments are stress-specific and correspond to adaptiv...

journal_title：Journal of experimental botany

authors： Reymond M,Muller B,Tardieu F

更新日期：2004-11-01 00:00:00

abstract：:If, following an inductive treatment of 2 d of continuous darkness, shoot apices of Pharbitis nil are cultured 1 d later on White's medium supplemented with 2% sucrose, they cannot form carpels, but they can if they are cultured on 2% glucose. It was hypothesized that the differential effect of these sugars was becaus...

journal_title：Journal of experimental botany

authors： Parfitt D,Herbert RJ,Rogers HJ,Francis D

更新日期：2004-10-01 00:00:00

abstract：:A cDNA sequence coding for a pea (Pisum sativum L.) 2-Cys peroxiredoxin (2-Cys Prx) has been cloned. The deduced amino acid sequence showed a high sequence homology to the 2-Cys Prx enzymes of Phaseolus vulgaris (86%), Arabidopsis thaliana (75%), and Spinacia oleracea (75%), and contained a chloroplast target sequence...

journal_title：Journal of experimental botany

authors： Bernier-Villamor L,Navarro E,Sevilla F,Lázaro JJ

更新日期：2004-10-01 00:00:00